Bacillus cereus Enterotoxin T Gene (bcET) Probe qPCR Kit Other ProteinsDescription Bacillus cereus Enterotoxin T Gene (bcET) Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe based real time PCR. Purified DNA is directly amplified using sequence specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real time monitoring of amplification. The resulting signals may be used for
and other biological samples Sample Amount 100 μL Sensitivity 74
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue or UBE2J2 antigen-treated (Neutralization experiment) using P109095(UBE2J2 Antibody) at dilution 1/80
Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue or MYDGF antigen-treated (Neutralization experiment) using P107608(MYDGF Antibody) at dilution 1/25
Mar 2008] Swissprot No Q6PGQ7 Gene Accession 79866 WB Predicted band size 61 kDa WB Positive control WB Recommended dilution 500-2000 IHC predicted cell location nan IHC positive control nan IHC Recommed dilution nan Storage
Q4: Is this product safe for clinical or diagnostic applications
003 pg/µL (ST0 stock: 3000 pg/µL) Reaction volume 30 µL total reaction volume (20 µL qPCR mix + 10 µL sample or control) qPCR mix per reaction qPCR Reaction Buffer 17 µL + NS0&SP2/0 Primer & Probe MIX 3 µL = 20 µL per reaction Thermal cycling conditions Activation: 95 °C 10:00 (1 cycle)
and high-throughput screening studies
Detection limit (LOD) Not more than 35 CFU/reaction Species coverage Covers ~92% of known fungal species (approximately 5
Rhizoma corydalis Probe qPCR Kit User Manual
microsection tissues
Protein gel coagulates quickly
the HB105-S1 ensures safe and reliable operation
